General description

Native trypsin from human pancreas. Serine protease that specifically hydrolyzes amides, peptides, and proteins at the C-terminus side of arginine and lysine residues.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Merck Index13, 9865.Travis, J., and Roberts, R.C. 1969. Biochemistry 8, 2884.

Packaging

50 µg in Plastic ampoule

Physical form

Salt-free.

Preparation Note

Prepared from tissue that has been shown by certified tests to be negative for HBsAg and for antibodies to HIV and HCV.

Reconstitution

Following reconstitution, aliquot and freeze (-70°C).

Unit Definition

One unit is defined as the amount of enzyme that will hydrolyze 1 µmol of N-benzyl-L-arginine-pNA per min at 25°C, 200 mM Tris-HCl, pH 7.8, 20 mM CaCl₂.

Warning

Toxicity: Irritant (B)